Our turnaround time depends on project size (e.g. number ofsamples and the number of analytes to be studied), butmost projects are completed in 2-3 weeks.
We can interrogate ~100 analytes from ~10,000 cells. To put this in perspective, from clinical biopsies (18-gauge needles with 30% tumor on the slide), this amounts to 5-6 8 micron tissue sections.
We work with a wide range of sample inputs: cell pellets, isolated extracellular vesicles, isolated PBMCs, cell line- and patient- derived xenografts, core needle biopsies and fine needle aspirates.
Additionally, we work with both frozen and formalin-fixed paraffin embedded samples, so please ask our scientists how best to prepare your samples for RPPA analysis.
Yes, we can investigate the sorted cells.
We have no preference on whether the samples are frozen or FFPE, and the choice depends on client’s project and objectives.
Yes, we can customize the proteins/pathway to be studied according to your needs. We have a list of 600+ proteins that can be chosen from.
Yes, we can use a client’s antibody or a client recommended antibody provided it passes our internal validation process.
Typically, we normalize the protein intensity values to the total protein content within each sample. However, if requested, we can normalize data to GAPDH, β-actin, TATA binding protein or a protein of your choosing provided there is a RPPA validated antibody against that protein.
This highly depends on the project and the discussion with your scientific team. We can set up a call to discuss the best statistical approach for your project.
Our quantitation is relative. For some proteins where a highly purified recombinant protein is available, we can provide absolute quantitative measurements.
We typically print samples in technical triplicates. For cell culture and preclinical CDX/PDX models, we recommend 3-6 biological replicates.
CVs generally range from 2-10% for technical replicates.
Each sample in printed in a technical triplicate onto the RPPA. If the coefficient of variation of the sample for a given analyte falls above a pre-specified value, it is removed from analysis.
Every sample printed onto the array is checked for quality. Every array is printed with positive controls for the analyte of interestto ensure stain quality. For clinical specimens, every sample is reviewed by our own pathologist.
Data will be sent over by secure email or data can be accessed via secure log-in through our LIMS system.
Raw data statistics and final normalized data for each sample will be delivered in a data table. If requested, preliminary analysis of the data such as principal component analysis, two-way unsupervised hierarchical clustering, and individual protein analyses(e.g. bar charts) will be performed.
Yes, our lab is CLIA certified.
Please refer to our shipping recommendationsto ensure your samples are best preserved during the shipping process. Please ship ‘preferredo vernight’ to:
Theralink Technologies, Inc.
15000 West 6th Avenue
Golden, CO 80401